Colon cancer-associated B2 Escherichia coli colonize gut mucosa and promote cell proliferation

Figure 2 Ability of B2 Escherichia coli strains to form biofilm. Biofilm formation of colon cancer-associated and diverticulosis-associated B2 Escherichia coli (E. coli) were compared to that of the non-pathogenic K-12 E. coli strain C600 and the biofilm producer AIEC strain LF82. A: Biofilm formation on abiotic surface. Results are expressed as specific biofilm formation (SBF) index; B: Biofilm formation on human I-407 intestinal epithelial cells. E. coli strain 11G5 was isolated from a patient with colon cancer and E. coli strain 12H1 from a patient with diverticulosis. Bacteria were stained using goat anti-E. coli polyclonal antibodies (green) and I-407 cells were labeled for actin cytoskeleton using TRITC-labeled phalloidin (red). Y- and Z-stack projections are presented.