Detection of promoter hypermethylation of Wnt antagonist genes in fecal samples for diagnosis of early colorectal cancer

doi:10.3748/wjg.v20.i20.6329

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May 28, 2014 (publication date) through Apr 27, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 28, 2014; 20(20): 6329-6335
Published online May 28, 2014. doi: 10.3748/wjg.v20.i20.6329

Table 2 SFRP2 and WIF-1 gene primer sequences for methylation-specific polymerase chain reaction assays

Gene	Primer (5’-3’)	Product size (bp)	Annealing temperature (°C)
M: SFRP2	GGGTCGGAGTTTTTCGGAGTTGCGC	138	62
	CCGCTCTCTTCGCTAAATACGACTCG		(38)
U: SFRP2	TTTTGGGTTGGAGTTTTTTGGAGTTGTGT	145	52
	AACCCACTCTCTTCACTAAATACAACTCA		(38)
M: WIF-1	GGGCGTTTTATTGGGCGTAT	197	58
	AAACCAACAATCAACGAAC		(38)
U: WIF-1	GGGTGTTTTATTGGGTGTAT	198	55
	AAACCAACAATCAACAAAAC		(38)

SFRP: Secreted frizzled related protein; WIF-1: Wnt inhibitory factor-1.

Citation: Zhang H, Zhu YQ, Wu YQ, Zhang P, Qi J. Detection of promoter hypermethylation of Wnt antagonist genes in fecal samples for diagnosis of early colorectal cancer. World J Gastroenterol 2014; 20(20): 6329-6335
URL: https://www.wjgnet.com/1007-9327/full/v20/i20/6329.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i20.6329