Is hepatitis B-virucidal validation of biocides possible with the use of surrogates?

Table 3 Information on the stability of hepatitis B virus published by the World Health Organization[99]

Biocide/measure	Concentration/temperature	Contact time	Remarks
Sodium hypochlorite	0.25%	3 min	Antigenicity of hepatitis B surface antigen is destroyed, infectivity is probably destroyed
Sodium hypochlorite	5%	10 min	Inactivation of virus
Glutaraldehyde	2% (room temperature)	5 min	Inactivation of virus
Glutaraldehyde	2% (98  °C)	2 min	Inactivation of virus
Formaldehyde	5%	2 min	Inactivation of virus
Isopropanol	70%	2 min	Inactivation of virus
Ethanol	80% (11  °C)	2 min	Inactivation of virus
Autoclaving	121  °C	20 min	Lost of infectivity
Heat sterilization	160  °C	1 h	Lost of infectivity