Proteomic analysis of liver mitochondria from rats with nonalcoholic steatohepatitis

Table 2 Dysregulated proteins in liver mitochondria of nonalcoholic steatohepatitis rats

	Spot no.	Protein GI	Protein name	Fold change	GO molecular function
Up-regulated	611	157822175	dehydrogenase 1 β subcomplex 10 (Ndufb10)	3.05	metabolic process/electron transport/ATP synthesis
	605	257467641	Suppressor of glucose by autophagy (SOGA)	2.97	suppressing glucogenesis
	510	29436756	Nuclear mitotic apparatus protein 1	2.68	microtubule binding/tubulin binding
	220	76779273	Hspd1 protein	2.54	ATP binding/insulin binding
	349	149029483	Mitochondrial F1 complex, alpha subunit, isoform 1	2.49	ATP synthesis/ADP binding
	51	202549	Iodothyronine 5' monodeiodinase	2.37	deiodination of thyroxine to T3
	135	148676986	Phosphoglucomutase 5	1.81	carbohydrate metabolism/Ca2+ homeostasis
	35	344244087	UDP-glucuronosyl-transferase 2B31	1.65	transferase activity/transferring hexosyl groups
Down-regulated	310	158631196	Peptidyl-prolyl cis-trans isomerase H isoform 2	-106	isomerase activity/ribonucleoprotein complex binding
	208	225690572	Leucine-rich repeat- containing protein 49 isoform 3	-106	protein-protein interaction/component of immune system
	183	300796253	Mitochondrial 10-formyltetrahydrofolate dehydrogenase (ALDH1L2)	-106	folate metabolism/CO production
	221	123244269	Microtubule-actin crosslinking factor 1	-106	ATPase activity/calcium ion binding/microtubule binding
	132	148687612	DEAH (Asp-Glu-Ala-His) box polypeptide 37, isoform CRA_b	-3.88	ATP binding/helicase and hydrolase activity
	248	60688189	Agmat protein	-2.85	agmatinase activity/hydrolase activity/metal ion binding
	244	351700869	Zinc finger protein 613	-2.52	nucleic acid binding/zinc ion binding
	258	47087119	Estradiol 17-β-dehydrogenase 8	-2.24	3-hydroxyacyl-CoA dehydrogenase activity/estradiol 17-β-dehydrogenase activity/oxidoreductase activity/testosterone 17-β-dehydrogenase (NAD+) activity
	122	6679299	Prohibitin	-2.14	histone deacetylase binding/transcription regulatory region DNA binding
	237	8393186	carbamoyl-phosphate synthase	-1.88	ATP binding/calcium ion binding/carbamoyl-phosphate synthase (ammonia) activity
	234	20304123	3-mercaptopyruvate sulfurtransferase	-1.87	3-mercaptopyruvate sulfurtransferase activity/thiosulfate sulfurtransferase activity/transferase activity
	179	149027156	Mitochondrial 3-oxoacyl-coenzyme A thiolase, isoform CRA_f	-1.85	acetyl-CoA C-acyltransferase activity/transferase activity
	260	148747393	Trifunctional enzyme subunit, alpha, mitochondrial precursor (Hadha)	-1.76	3-hydroxyacyl-CoA dehydrogenase activity
					NAD binding/enoyl-CoA hydratase activity/fatty-acyl-CoA binding/long-chain-3-hydroxyacyl-CoA dehydrogenase activity
	156	347800699	T-complex protein 1 subunit theta	-1.74	ATP binding/nucleotide binding/unfolded protein binding
	257	11968102	Ornithine aminotransferase, mitochondrial precursor	-1.60	2-oxoglutarate 5-5-aminotransferase activity/ornithine-oxo-acid transaminase activity/pyridoxal phosphate binding
	206	351715881	GRIP and coiled-coil domain	-1.55	protein binding-containing protein 2

The -10⁶-fold change denotes very low expression in the NASH group compared with the control group. GIs in bold italics are proteins that are known to be located exclusively in mitochondria. Underlined proteins are known to be located in the inner mitochondrial membrane. ATP: Adenosine triphosphate (ATP); GO: Gene ontology.