Clinicopathological study of primary biliary cirrhosis with interface hepatitis compared to autoimmune hepatitis

doi:10.3748/wjg.v20.i13.3597

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Apr 7, 2014; 20(13): 3597-3608
Published online Apr 7, 2014. doi: 10.3748/wjg.v20.i13.3597

Table 2 Primary antibodies used in this study

Primary antibody	Source	Clone	Animal	Type	Pretreatment	Dilution
CD3	Nichirei	PS1	Mouse	Mono	MW³	1:1
CD4	Nichirei	4B12	Mouse	Mono	MW³	1:1
CD8	Dako	C8/144B	Mouse	Mono	MW¹	1:20
CD20	Dako	L26	Mouse	Mono	-	1:400
CD38	Epitomics	EPR4106	Rabbit	Mono	AC²	1:800
CD138	Dako	ML15	Mouse	Mono	MW³	1:100
IgG	Dako	A57H	Mouse	Mono	-	1:200
IgM	Nichirei	R1/69	Mouse	Mono	MW³	1:1
IgA	Novocastra	N1CLA	Mouse	Mono	PK	1:300

¹Citrate, pH = 6.0;

²Ethylenediaminetetraacetic acid (EDTA) buffer, pH8.0;

³ Tris EDTA buffer, pH = 9.0. MW: Microwave; AC: Autoclave; PK: Proteinase K, pH = 7.5 (Dako).

Citation: Kobayashi M, Kakuda Y, Harada K, Sato Y, Sasaki M, Ikeda H, Terada M, Mukai M, Kaneko S, Nakanuma Y. Clinicopathological study of primary biliary cirrhosis with interface hepatitis compared to autoimmune hepatitis. World J Gastroenterol 2014; 20(13): 3597-3608
URL: https://www.wjgnet.com/1007-9327/full/v20/i13/3597.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i13.3597