ATG16L1 and NOD2 polymorphisms enhance phagocytosis in monocytes of Crohn&rsquo;s disease patients

doi:10.3748/wjg.v20.i10.2664

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 14, 2014; 20(10): 2664-2672
Published online Mar 14, 2014. doi: 10.3748/wjg.v20.i10.2664

Table 2 Designed primers and restriction enzymes for genotyping by restriction fragment length polymorphisms

Gene	Forward	Reverse	Restriction enzyme
ATG16L1	GGTACCCTCACTTCTTTACCAGAA CCAGGAAGAG	TGGAGTCCACAGGTTAGTGTGCAGGAGAGTAAGG	Sap1
rs2241880	GGTACCCTCACTTCTTTACCAGAA CCAGGAAGAG	TGGAGTCCACAGGTTAGTGTGCAGGAGAGTAAGG	Sap1
IRGM	CCCGTGTCGTACCCAAGCAGAGTGTGCTTGAAGA	CTTTACCATTGTACTCCTTGTGCCCAGCAGGTG	MboI
rs13361189	CCCGTGTCGTACCCAAGCAGAGTGTGCTTGAAGA	CTTTACCATTGTACTCCTTGTGCCCAGCAGGTG	MboI
NOD2	ATGTGTCTAAGGGACAGGTG	AACTGAGGTTCGGAGAGCTA	NlaIV
rs2066847
rs5743293

rs2066847 and rs5743293 are the same single-nucleotide polymorphism (SNP) in nucleotide-binding ligomerization domain-containing protein 2 (NOD2). ATG16L1: Autophagy related like 1; IRGM: Immunity-related GTPase family M protein.

Citation: Wolfkamp SC, Verseyden C, Vogels EW, Meisner S, Boonstra K, Peters CP, Stokkers PC, te Velde AA. ATG16L1 and NOD2 polymorphisms enhance phagocytosis in monocytes of Crohn’s disease patients. World J Gastroenterol 2014; 20(10): 2664-2672
URL: https://www.wjgnet.com/1007-9327/full/v20/i10/2664.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i10.2664