Clinicopathological and biological significance of cripto overexpression in human colon cancer

Figure 5 Effects of S1 treatment on matrix metalloproteinase-7 and -9 expression of colon cancer line LS-174T. After cancer cells were treated with S1 siRNA for different times, cells were harvested, and total RNA and proteins were extracted. Real-time reverse transcription-polymerase chain reaction and western blotting were performed to detect mRNA and protein levels, respectively, of matrix metalloproteinase (MMP)-7 and -9. A: Expression level of mRNA and protein of MMP-7 (2 h); B: Expression level of mRNA and protein of MMP-9 (72 h). 1: Scr siRNA; 2: S1(25 nmol/L).