P115 promotes growth of gastric cancer through interaction with macrophage migration inhibitory factor

Figure 4 P115-shRNA inhibited the secretion and expression of macrophage migration inhibitory in culture supernatant and cells. A: Macrophage migration inhibitory factor (MIF) in BGC cells was extracted through protein precipitation with a multiple clone antibody, and P115 was detected in the protein complex by Western blotting; B: 48 h after transfection with 2 μg P115-shRNA, the secretion of MIF into the supernatant was inhibited as measured by ELISA. MIF in cells was reduced as measured by real-time PCR (C) and Western blotting (D). β-actin was used as a loading control for Western blotting and glyceraldehyde 3-phosphate dehydrogenase was used as an internal control for PCR. Data are mean ± SD of three experiments.