Events associated with apoptotic effect of p-Coumaric acid in HCT-15 colon cancer cells

Figure 3 Events associated with growth-inhibitory effect of p-Coumaric acid. A: Human colorectal carcinoma (HCT-15) cells were treated with p-Coumaric acid for specified time-periods and then mitochondrial membrane potential were determined using rhodamine-123 by flow cytometry. Mean differences are significant at 6 and 12 h compared with untreated control cells (P < 0.05 vs untreated control cells); B: HCT 15 cells were treated with p-Coumaric acid and evaluated using merocyanine 540 to quantify the lipid layer breaks (LLBs). Data is representative of three independent experiments and mean differences are significant at 3 and 6 h compared with untreated control cells (P < 0.05 vs untreated control cells). MMP: Mitochondrial membrane potential.