Reference genes for quantitative RT-PCR data in gastric tissues and cell lines

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Nov 7, 2013; 19(41): 7121-7128
Published online Nov 7, 2013. doi: 10.3748/wjg.v19.i41.7121

Table 2 Summary of five reference genes and a target gene

Symbol	Gene name (Assay ID¹)	Location	Description	Efficiency
ACTB	β-Actin	7p22	Cytoskeletal structural protein	109%
ACTB	(Hs03023943_g1)	7p22	Cytoskeletal structural protein	109%
B2M	β-2-Microglobulin	15q21	Beta-chain of major histocompatibility complex class I molecules	104%
B2M	(Hs00984230_m1)	15q21		104%
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	12p13	Oxidoreductase in glycolysis and gluconeogenesis	102%
GAPDH	(Hs99999905_m1)	12p13	Oxidoreductase in glycolysis and gluconeogenesis	102%
RPL29	Ribosomal protein L29	3p21	Structural constituent of the ribosome	100%
RPL29	(Hs00426490_g1)	3p21	Structural constituent of the ribosome	100%
18S rRNA	18S ribosomal RNA	22p12	Ribosome subunit	110%
18S rRNA	(Hs99999901_s1)	22p12	Ribosome subunit	110%
DNMT1	DNA (cytosine-5-)-methyltransferase 1	19p13	Regulation of tissue-specific patterns of methylated cytosine residues	98%
DNMT1	(Hs00945875_m1)	19p13		98%

¹TaqMan^® probes were purchased as Assays-on-Demand Products for Gene Expression (Life Technologies, Foster City, CA).

Citation: Wisnieski F, Calcagno DQ, Leal MF, Santos LCD, Gigek CO, Chen ES, Pontes TB, Assumpção PP, Assumpção MB, Demachki S, Burbano RR, Smith MAC. Reference genes for quantitative RT-PCR data in gastric tissues and cell lines. World J Gastroenterol 2013; 19(41): 7121-7128
URL: https://www.wjgnet.com/1007-9327/full/v19/i41/7121.htm
DOI: https://dx.doi.org/10.3748/wjg.v19.i41.7121

Wisnieski F, Calcagno DQ, Leal MF, Santos LCD, Gigek CO, Chen ES, Pontes TB, Assumpção PP, Assumpção MB, Demachki S, Burbano RR, Smith MAC. Reference genes for quantitative RT-PCR data in gastric tissues and cell lines. World J Gastroenterol 2013; 19(41): 7121-7128 [PMID: 24222956 DOI: 10.3748/wjg.v19.i41.7121]

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