Reference genes for quantitative RT-PCR data in gastric tissues and cell lines

Table 2 Summary of five reference genes and a target gene

Symbol	Gene name (Assay ID1)	Location	Description	Efficiency
ACTB	β-Actin	7p22	Cytoskeletal structural protein	109%
	(Hs03023943_g1)
B2M	β-2-Microglobulin	15q21	Beta-chain of major histocompatibility complex class I molecules	104%
	(Hs00984230_m1)
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	12p13	Oxidoreductase in glycolysis and gluconeogenesis	102%
	(Hs99999905_m1)
RPL29	Ribosomal protein L29	3p21	Structural constituent of the ribosome	100%
	(Hs00426490_g1)
18S rRNA	18S ribosomal RNA	22p12	Ribosome subunit	110%
	(Hs99999901_s1)
DNMT1	DNA (cytosine-5-)-methyltransferase 1	19p13	Regulation of tissue-specific patterns of methylated cytosine residues	98%
	(Hs00945875_m1)

¹TaqMan^® probes were purchased as Assays-on-Demand Products for Gene Expression (Life Technologies, Foster City, CA).