Mesenchymal stem cells alleviate TNBS-induced colitis by modulating inflammatory and autoimmune responses

Figure 9 Changes in mRNA expression and colonic protein levels of Tregs-related inflammatory mediators following treatment with bone marrow mesenchymal stem cells. A, B: Real-time polymerase chain reaction. No significant changes in mRNA expressions of transforming growth factor (TGF)-β and Foxp3 were observed when compared to controls (P > 0.05). While the mRNA expressions of TGF-β and Foxp3 increased after BMSCs transplantation (P < 0.01); C, D: Western blotting. The colonic protein level of TGF-β and Foxp3 were confirmed to show the same results as real-time polymerase chain reaction. ^cP < 0.05, ^dP < 0.01 vs TNBS-PBS. TNBS-MSC: Trinitrobenzene sulfonic acid-mesenchymal stem cells; TNBS-PBS: Trinitrobenzene sulfonic acid-phosphate-buffered saline.