Influence of up-regulation of Notch ligand DLL4 on biological behaviors of human gastric cancer cells

Figure 4 Effect of Delta-like ligand 4 up-regulation on the secretion and activation of extracellular matrix metalloproteinases. Twenty microlitres of culture supernatant from each group was used. Coomassie Blue R-250 staining of the zymogram revealed significantly increased matrix metalloproteinases (MMP)-2 proenzyme but decreased MMP-9 proenzyme in the SGC7901-Delta-like ligand 4 (DLL4) group vs the SGC7901-vector group. SGC7901-vector: SGC7901 cells transfected with an empty vector; SGC7901-DLL4: SGC7901 cells transfected with vector encoding human DLL4 gene; Control: Non-transfected SGC7901 cells.