Reversal of multidrug resistance in gastric cancer cells by CDX2 downregulation

Figure 1 mRNA and protein expressions of CDX2 after RNA interference. A, B: Expression level of CDX2 mRNA was determined by semiquantitative reverse-transcriptase polymerase chain reaction; C, D: Expression level of CDX2 protein was determined by Western blotting. mRNA results were expressed as the ratio of CDX2 to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Western blotting results were expressed as the ratio of optical density of CDX2 bands to GAPDH bands. All values are mean ± SE. ^aP < 0.05 for SGC7901/DDP + small interfering RNA (siRNA)-CDX2 cells vs SGC7901/DDP + siRNA-NC cells and SGC7901/DDP cells. Lane 1: SGC7901/DDP + siRNA-CDX2 cells; Lane 2: SGC7901/DDP + siRNA-NC cells; Lane 3: SGC7901/DDP cells.