Hugl-1 induces apoptosis in esophageal carcinoma cells both in vitro and in vivo

Figure 2 Effect of Hugl-1 on cell cycle distribution of Eca109 cells in vitro. A: Cells were treated with pEZ-M29-Hugl1, pEZ-M29-eGFP or phosphate buffered saline (PBS) for 48 h and were then prepared for fluorescence-activated cell sorting analysis; B: Data are presented as mean ± SD of three independent experiments; C: Western blotting data of p21 and cyclin D1 protein expression levels following transfection with pEZ-M29-Hugl1 or controls; D: Analysis of the expression of proteins. ^aP < 0.05 vs the pEZ-M29-eGFP-treated and PBS-treated groups. GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.