Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease

Figure 1 Agarose gel electrophoresis of multiplex polymerase chain reaction amplification products from Enterococcus species isolates. The reference strain Enterococcus faecalis (E. faecalis) ATTC29212 (EF reference strain) possessing the genes cylA, asa1 and gelE constituted the positive control. The negative control was a reference strain of Enterococcus faecium (E. faecium) ATTC 35667 Efm reference strain that did not possess any of the examined genes. Product sizes were as follows, asa1: 375 bp; gelE: 213 bp; cylA: 688 bp; esp: 510 bp; hyl: 276 bp. EF: Tested strains of E. faecalis; Efm: Tested strains of E. faecium; M: Mass marker 100-1000 bp.