Bone-marrow mesenchymal stem cells reduce rat intestinal ischemia-reperfusion injury, ZO-1 downregulation and tight junction disruption via a TNF-α-regulated mechanism

Table 1 Grade of intestinal mucosal injury after intestinal ischemia/reperfusion in the different groups

Group	Chiu’s score
	2 h	6 h	24 h	72 h	144 h
Sham group	0.5 ± 0.8	0.5 ± 0.5	0.7 ± 0.8	0.3 ± 0.8	0.7 ± 0.8
NS + I/R injury	18.0 ± 3.9b	27.8 ± 2.3b	23.7 ± 5.2b	19.0 ± 3.8b	11.0 ± 3.0b
BM MSCs + I/R injury	13.7 ± 3.3bd	15.2 ± 2.9bd	13.7 ± 1.5bd	8.3 ± 2.3bd	5.8 ± 2.6bd
Anti-TNF-α + I/R injury		6.5 ± 1.2df
Anti-TNF-αR1-IgG + I/R injury		7.7 ± 1.2df

All values are mean ± SD (n = 6, three paraffin sections were prepared from each tissue sample. Two pathologists who were blinded to the source of the slides analyzed each slide);

^bP < 0.01 vs the Sham group,

^dP < 0.01 vs the saline (NS) + ischemia/reperfusion (I/R) injury group,

^fP < 0.01 vs bone-marrow mesenchymal stem cells (BM MSCs) + I/R injury group. TNF-α: Tumor necrosis factor-α.