Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease

Table 3 Presence of asa1 gene, degree of adhesion to human gut epithelium cells and biofilm production among Enterococcus strains

Source	Strains	asa1	Adherence	Biofilm production (after 48 h) A492 nm± SD
Crohn's disease	Enterococcus faecalis 12A/1	-	+++	0.995 ± 0.351
	Enterococcus faecalis 31B	-	+++	1.302 ± 0.585
	Enterococcus faecalis 51A/1	-	++	1.083 ± 0.413
	Enterococcus faecium 3A	-	-	1.286 ± 0.088
	Enterococcus faecium 57A	+	+++	0.698 ± 0.066
	Enterococcus faecium 19B	+	+++	1.087 ± 0.368
	Enterococcus avium 51A/2	-	-	2.101 ± 0.424
	Enterococcus avium 25B	-	-	2.474 ± 0.683
	Enterococcus avium 10A	-	-	1.423 ± 0.284
Ulcerative colitis	Enterococcus faecalis 35A	+	+++	1.528 ± 0.326
	Enterococcus faecalis 48A	-	+++	0.767 ± 0.582
	Enterococcus faecalis 77A	-	++	0.822 ± 0.196
	Enterococcus faecalis 32B	+	++	0.557 ± 0.344
	Enterococcus avium 40A	-	+++	0.859 ± 0.152
	Enterococcus avium 34A/2	-	-
	Enterococcus avium 40B	-	-	0.282 ± 0.078
Control	Enterococcus faecalis 5B	-		0.512 ± 0.065
	Enterococcus faecalis 27B/1	-	-	0.934 ± 0.103
	Enterococcus faecalis 46B	-	++	0.507 ± 0.095
	Enterococcus faecalis 55B	-	-	0.408 ± 0.242
	Enterococcus faecalis 66B	-	-	0.172 ± 0.031
	Enterococcus faecium 27B/2	-		0.917 ± 0.269
	Enterococcus faecium 30B	-	-	0.359 ± 0.087
	Enterococcus avium 15B/1	-	-
	Enterococcus avium 16B/1	-	-	0.305 ± 0.104
	Enterococcus avium 27B/3	-	-	0.657 ± 0.182
	Enterococcus durans 15B/2	-	-
	Enterococcus durans 16B/2	-	-	0.315 ± 0.246
ATCC control	Enterococcus faecalis 29212	+	+++	0.877 ± 0.221
	Enterococcus faecium 35667	-	++	0.722 ± 0.371

Adhesion degree was assessed as +++ (strong adhesive abilities); ++ (average adhesive abilities) and - (lack of adhesive abilities). Biofilm formation was measured in triplicate at two time points (0 and 48 h). The differences between the time points were averaged, and the mean ± SD are shown.