Fluctuations in butyrate-producing bacteria in ulcerative colitis patients of North India

Table 4 Enumeration of butyrate producers in fecal samples through fluorescent in situ hybridization-flow cytometry (mean ± SE)

Cluster or species	Out of total microbiota	Species	Out of respective cluster
Control fecal samples
C. coccoides cluster	25.69% ± 1.62%	R. intestinalis	14.48% ± 1.52%
		E. hallii	5.93% ± 0.54%
C. leptum cluster	13.74% ± 1.05%	F. prausnitzii	11.66% ± 1.55%
R .intestinalis1	3.71% ± 0.39%
E. hallii1	1.52% ± 0.13%
F. prausnitzii1	1.60% ± 0.21%
UC fecal samples (moderate)
C. coccoides cluster	12.70% ± 4.60%	R. intestinalis	10.00% ± 2.25%
		E. hallii	4.03% ± 0.3%
C. leptum cluster	6.40% ± 3.40%	F. prausnitzii	7.5% ± 2.3%
R .intestinalis1	1.27% ± 0.10%
E. hallii1	0.51% ± 0.01%
F. prausnitzii1	0.48% ± 0.14%
UC fecal samples (severe)
C. coccoides cluster	9.80% ± 2.40%	R. intestinalis	9% ± 1.83%
		E. hallii	4.2% ± 0.2%
C. leptum cluster	6.20% ± 1.80%	F. prausnitzii	6.01% ± 1.6%
R .intestinalis1	0.82% ± 0.04%
E. hallii1	0.41% ± 0.01%
F. prausnitzii1	0.37% ± 0.02%
UC fecal samples (remission)
C. coccoides cluster	12.99% ± 2.65%	R. intestinalis	11.40% ± 1.83%
		E. hallii	5.07% ± 0.6%
C. leptum cluster	7.89% ± 1.50%	F. prausnitzii	7.40% ± 2.32%
R .intestinalis1	1.48% ± 0.04%
E. hallii1	0.65% ± 0.01%
F. prausnitzii1	0.58% ± 0.03%

¹Abundance of Roseburia intestinalis (R. intestinalis), Eubacterium hallii (E. hallii) and Fecalibacterium prausnitzii (F. prausnitzii) were calculated out of total microbiota. Clostridium coccoides (C. coccoides) and Clostridium leptum (C. leptum) were enumerated as proportion of total bacterial cell hybridized with group-specific probe. R. intestinalis, E. hallii and F. prausnitzii were enumerated as proportion of cell hybridized with species-specific probe. UC: Ulcerative colitis.