MAWBP and MAWD inhibit proliferation and invasion in gastric cancer

Figure 5 Effect of mitogen-activated protein kinase activator with WD40 repeats binding protein and mitogen-activated protein kinase activator with WD40 repeats on expression of biomarkers specific for epithelial-mesenchymal transition induced by transforming growth factor-β1. A: According to the expression of transforming growth factor (TGF)-β downstream reporter gene plasminogen activator inhibitor (PAI)-1, the optimum TGF-β1 concentration and time were confirmed as 4 ng/mL for 24 h; B: SGC7901 cells overexpressing mitogen-activated protein kinase activator with WD40 repeats (MAWD)/MAWD binding protein (MAWBP) were stimulated by 4 ng/mL TGF-β1 for 24 h. Cells that overexpressed both MAWBP and MAWD displayed a pebble-like shape, while vector cells showed a classical mesenchymal phenotype (original magnification, ×400); C: Expression of E-cadherin was strongest in the MAWBP/D cotransfection group and weakest in the vector group, using Western blotting. Snail and N-cadherin were inversely associated with E-cadherin expression.