Twist2 is a valuable prognostic biomarker for colorectal cancer

Table 3 Univariate and multivariate analysis of the prognostic factors for overall survival

Prognostic factors	Univariate analysis			Multivariate analysis
	HR	95%CI	P value	HR	95%CI	P value
Gender
Female	1
Male	1.011	0.490-2.085	0.977	NA	NA	NA
Age
< 59	1
≥ 59	1.579	0.750-3.326	0.229	NA	NA	NA
T-stage
T1-2	1
T3-4	1.118	0.493-2.534	0.789	NA	NA	NA
N-stage
N0	1
N1-2	2.172	1.056-4.468	0.035	NS	NS	NS
M-stage
M0	1			1
M1	6.324	2.659-15.041	< 0.001	7.694	2.927-20.224	< 0.001
Tumor differentiation
Moderate/good	1
Poor	1.290	0.491-3.384	0.229	NA	NA	NA
Vascular invasion
No	1
Yes	3.398	1.601-7.211	0.001	NS	NS	NS
Tumor location
Rectum	1
Colon	1.188	0.517-2.729	0.685	NA	NA	NA
CEA level (ng/mL)
≤ 5	1
> 5	3.173	1.475-6.827	0.003	NS	NS	NS
Twist2 expression
Negative	1			1
Positive	4.964	1.181-20.863	0.029	5.744	1.347-24.298	0.018

Multivariate analysis included adjustment for N-stage, M-stage, vascular invasion, serum carcinoembryonic antigen level and Twist2 expression. T: Tumor; N: Node; M: Metastasis; HR: Hazard ratio; NA: Not available; NS: Not significant.