Heme status affects human hepatic messenger RNA and microRNA expression

Figure 2 Pathway analysis illustrating differential gene expression in iron protoporphyrin- vs 4, 6-dioxoheptanoic acid- treated Huh-7 cells. Cells were cultured and exposed to iron protoporphyrin (10 μmol/L) or 4, 6-dioxoheptanoic acid (500 μmol/L) for 6 h, after which they were harvested and total RNA isolated using TRIzol. Pathway analysis was performed using Ariadne Pathway Studio by defining sub-networks of genes selected using gene set enrichment analysis (P≤ 0.01). The resulting sub-networks were combined. Entities satisfying the filtering criteria are depicted. The intensity of red or blue of the entities themselves indicates the corresponding degree of up- or down-regulation, respectively. Differing shapes are used to represent the entity types and the relationships among them.