Effects of resistin-like molecule β over-expression on gastric cancer cells in vitro

Figure 6 Over-expression of resistin-like molecule β decreased the expression of vascular endothelial growth factor, but not v-ets erythroblastosis virus E26 oncogene homolog 1, in gastric cancer cells. A: Western blotting indicated that 72 h after transfection, over-expression of resistin-like molecule β (RELMβ) abolished the expression of vascular endothelial growth factor (VEGF), but not v-ets erythroblastosis virus E26 oncogene homolog 1 (Ets1), in SGC-7901 and MKN-45 cells. Moreover, transfection of empty vector (mock) did not influence the expression of VEGF and Ets1; B: Reverse transcription polymerase chain reaction (RT-PCR) indicated the decreased VEGF transcription levels in SGC-7901 and MKN-45 cells transfected with pcDNA3.1-RELMβ for 72 h, but not in mock group. Moreover, the Est1 transcription levels were not influenced by transfection of pcDNA3.1-RELMβ or empty vector (mock); C: Real-time quantitative RT-PCR further demonstrated that transfection of pcDNA3.1-RELMβ for 72 h resulted in decreased transcription levels of VEGF, but not of Ets1, in SGC-7901 and MKN-45 cells. The symbol (a) indicates a significant decrease from parental cells (P < 0.01). Triplicate experiments were performed with essentially identical results. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.