Effects of resistin-like molecule β over-expression on gastric cancer cells in vitro

Figure 4 Over-expression of resistin-like molecule β decreased the expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 in gastric cancer cells. A: Western blotting indicated that 72 h after transfection, over-expression of resistin-like molecule β (RELMβ) abolished the expression of matrix metalloproteinase (MMP)-2 and MMP-9 in SGC-7901 and MKN-45 cells. However, transfection of empty vector (mock) did not influence their expression; B: Reverse transcription polymerase chain reaction (RT-PCR) indicated the decreased MMP-2 and MMP-9 transcription levels in SGC-7901 and MKN-45 cells transfected with pcDNA3.1-RELMβ for 72 h, but not in mock group; C: Real-time quantitative RT-PCR further demonstrated that transfection of pcDNA3.1-RELMβ for 72 h resulted in decreased transcription levels of MMP-2 and MMP-9 in SGC-7901 and MKN-45 cells. The symbol (a) indicates a significant decrease compared with parental cells (P < 0.01). Triplicate experiments were performed with essentially identical results. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.