Effects of resistin-like molecule β over-expression on gastric cancer cells in vitro

Figure 1 Transient transfection-mediated over-expression of resistin-like molecule β in gastric cancer cells. A: Western blotting indicated that low resistin-like molecule β (RELMβ) protein was detected in the parental SGC-7901 and MKN-45 cells, and transient transfection of the empty vector (mock) did not affect the expression levels of RELMβ. However, transient transfection of pcDNA3.1-RELMβ for 24 h, 72 h and 120 h resulted in increased RELMβ expression; B: 24 h, 72 h and 120 h after transfection, reverse transcription polymerase chain reaction (RT-PCR) indicated the increased RELMβ transcription levels in pcDNA3.1-RELMβ transfected SGC-7901 and MKN-45 cells, but not in mock group; C: Real-time quantitative RT-PCR further demonstrated that transfection of pcDNA3.1-RELMβ for 24 h, 72 h and 120 h resulted in upregulation of RELMβ transcription levels in SGC-7901 and MKN-45 cells. The symbol (a) indicates a significant increase compared with parental cells (P < 0.01). Triplicate experiments were performed with essentially identical results.