Germline mutation analysis of MLH1 and MSH2 in Malaysian Lynch syndrome patients

Table 1 List of primer sequences used for polymerase chain reaction amplification of the exons of MLH1 gene and their respective sizes of polymerase chain reaction products

MLH1 gene	Primer sequence (5’-3’)	Size (bp)	Ta (°C)
Exon 1	5’ cagagttgagaaatttgactgg 3’	339	50
	5’ taagtcgtagcccttaagtgag 3’
Exon 2	5’ tgtatgagcctgtaagacaaag 3’	315	50
	5’ tagtttccagaacagagaaagg 3’
Exon 3	5’ gggaattcaaagagatttgg 3’	373	50
	5’ gaggtctctgcaggtaaaatag 3’
Exon 4	5’ ctcatcgttgccacatatta 3’	319	50
	5’ gtactcaagatctctgccaaaa 3’
Exon 5	5’ tccccttgggattagtatctat 3’	306	50
	5’ cccacaaaagccaatagtcat 3’
Exon 6	5’ cactatcttaagacctcgcttt 3’	325	50
	5’ gctaaaccttgaccagaaacta 3’
Exon 7	5’ agaggagtctgtgttttgattc 3’	367	50
	5’ atcataaccttatctccaccag 3’
Exon 8	5’ atagtttgctggtggagataag 3’	306	50
	5’ agcctgtgtatttgactaaagc 3’
Exon 9	5’ agttagtttatgggaaggaacc 3’	438	50
	5’ aagaaccagactccaacagtc 3’
Exon 10	5’ atagtaagatagtgggctggaa 3’	432	62
	5’ tacctgtaagaagggacagaac 3’
Exon 11	5’ tatttgaacacactcagactcg 3’	476	62
	5’ taggaacaacagcacaatacc 3’
Exon 12	5’ atacagactttgctaccaggac 3’	475	50
	5’ agagagaagatgcaagtgattc 3’
Exon 13	5’ tgtcagataagcagtctaccag 3’	468	50
	5’ ttagtaaaggaagaggagcttg 3’
Exon 14	5’ cctcatttgtgttcgttttc 3’	411	50
	5’ gtcgaacttggatttgaaac 3’
Exon 15	5’ catgtttcagggattacttctc 3’	356	50
	5’ ggctaccaaatgactattttgc 3’
Exon 16	5’ gtatatgggggatgagtgttac 3’	319	50
	5’ cagaagtataagaatggctgtc 3’
Exon 17	5’ gtgctgtttgtcaggatgaa 3’	430	50
	5’ tgaatattagtggggatgagag 3’
Exon 18	5’ gtctgtgatctccgtttagaat 3’	358	50
	5’ ctacatcagcagatggaagtaa 3’
Exon 19	5’ caggacaccagtgtatgttg 3’	497	50
	5’ ctgtgacatgttcaagacctc 3’

bp: Base pair; Ta: Annealing temperature.