Study on RIZ1 gene promoter methylation status in human esophageal squamous cell carcinoma

Figure 6 Methylation-specific polymerase chain reaction electrophoresis for cancerous, neighboring and distal ending normal tissues. A: The results of electrophoresis for the polymerase chain reaction (MSP) products of positive retinoblastoma protein-interacting zinc finger gene 1 (RIZ1) methylation amplification sufferers in both their cancerous and neighboring normal tissues; B: The results of electrophoresis for the MSP products of sufferers in whose cancerous tissue positive RIZ1 methylation amplification appears while positive unmethylation amplification is present in the corresponding neighboring normal tissues. MA: Marker; M: Methylation (177bp); U: Unmethylation (175bp);1: Carcinomas; 2: Matched neighboring normal esophageal tissues; 3: Distal ending normal esophageal tissues; 4: Postive control for methylation; 5: Postive control for unmethylation; 6: Negative control.