Protection of ghrelin postconditioning on hypoxia/reoxygenation in gastric epithelial cells

Figure 4 Effects of D-Lys3-GHRP-6, capsazepine and LY294002 in ghrelin postconditioning on cell apoptosis in human gastric epithelial cells induced by hypoxia/reoxygenation. Cells were grouped as follows: normoxic culture for 6 h (A), 2 h hypoxia/4 h reoxygenation (B), DMSO vehicle postconditioning (C), ghrelin postconditioning (10^-7 mol/L) (D), D-Lys3-GHRP-6 + ghrelin postconditioning (E), capsazepine + ghrelin postconditioning (F), and LY294002 + ghrelin postconditioning (G). Subsequently, the cells were stained by annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI). Apoptosis was detected by flow cytometry.