Protection of ghrelin postconditioning on hypoxia/reoxygenation in gastric epithelial cells

Figure 2 Effects of D-Lys3-GHRP-6, capsazepine and LY294002 in ghrelin postconditioning on cell viability in human gastric epithelial cells induced by hypoxia/reoxygenation. Cells were grouped as follows: normoxic culture for 6 h (N), 2 h hypoxia/4h reoxygenation (H/R), DMSO vehicle postconditioning (DM), ghrelin postconditioning (10^-7 mol/L) (GH), D-Lys3-GHRP-6 + ghrelin postconditioning (D + GH), capsazepine + ghrelin postconditioning (C + GH) and LY294002 + ghrelin postconditioning (L + GH). Cell viability was detected by 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. mean ± SD. n = 6. ^bP < 0.01 vs H/R; ^cP < 0.05 vs GH.