Emerging roles of the ribonucleotide reductase M2 in colorectal cancer and ultraviolet-induced DNA damage repair

Figure 3 Effect of ribonucleotide reductase M2 on apoptosis and invasive capacity. A: Increased apoptosis following ribonucleotide reductase M2 (RRM2) mRNA inhibition by RNAi. Percentages of cells in the bottom left quadrant are live while cells to the right of the vertical line are apoptotic or dead; B: Quantifying bar graphs showing a significant increase in the percentage of apoptotic/dead cells following RRM2 mRNA knockdown with siRNA, ^aP < 0.05 vs negative control; ^cP < 0.05 vs blank control; C: Effect of RRM2 on invasive capacity of colorectal cancer cells. Invasion through the Matrigel-coated collagen insert after the indicated treatments is shown; D: Quantifying bar graphs showing a significant decrease in cell number following RRM2 mRNA knockdown with siRNA, ^bP < 0.01 vs negative control; ^dP < 0.01 vs blank control. Quad: Quadrant; UL: Upper left; UR: Upper right; LL: Lower left; LR: Lower right. PI: Propidium iodide.