Gene expression profiling and endothelin in acute experimental pancreatitis

Table 2 Up-regulated and down-regulated genes expressed¹

Group	Regulated genes	Abbreviation	Fold
	Up-regulated genes1
1	B-lymphocyte surface antigen		7.3
1	Endothelin-1	ET-1	3.2
1	Eosinophil chemotactic protein	ECP	11
1	T-cell surface glycoprotein CD5		2.6
1	Tumor necrosis factor	TNF-α	4
2	Laminin α 5	LAMA5	2.5
2	Matrix gla protein	MGP	7.4
2	Integrin α		27
3	Epithelial-cadherin	E-CADHERIN	3.1
3	Transmembrane ER-resident protein, type I	IRE1b	23.5
4	ATP binding cassette	ABC-transporter	23
4	C-C chemokine receptor type 8	C-C CKR-8, GPR-CY6	3.4
4	GABAA receptor	GABAA R	14.3
4	Glutamate receptor ionotropic, AMPA 1	GluR-1	9.1
4	Putative G protein-coupled receptor	GPR68 (OGR-1)	3.1
5	Calcium binding protein	CHP	2.6
5	Caveolin-1	CAV1	3.8
5	Iron response regulator	IRR	2.8
5	LDL receptor-related protein 6	LDL-R6	3.6
5	Insulin-like growth factor binding complex acid label chain precursor	ALS	2.7
5	Proto-oncogene tyrosine-protein kinase FES/FPS	C-FES	8
5	RAS GTPase-activating-like protein	RAN	3.9
5	Retinoic acid-inducible gene-1	RAIG1	6.4
5	Transforming growth factor-b	TGFb	2.8
5	Guanine nucleotide binding protein	G (Y) α-11	4.7
6	Nuclear factor of activated T cells	NF-ATc	3.5
6	Signal transducer and activator of transcription 5B	STAT5b	3
6	Transcription factor	TF	11.8
7	Glutathione peroxidase gastrointestinal	GSHPX_GI	2.5
7	Extracellular superoxide dismutase	EC-SOD	2.9
7	Heat shock related 70 kD proteins2		2.3
7	NADH dehydrogenase (ubiquinone)		2.3
7	Serum amine oxidase	SAO	2.2
8	ACE-related carboxypeptidase	ACE2	3.6
8	Aminopeptidase N	APN	2.3
8	ATP-dependent RNA helicase P54	DDX6	2
8	a enolase		2
8	a-Tryptase	TRYPTASE 1	2.6
8	cAMP cAMP-inhibited cGMP 3’5’-cyclic phosphodiesterase		9.4
8	Cholecystokinin type A receptor	CCK-AR	2.8
8	Dopamine β-hydroxylase	DBH	5.4
8	GAMMA-glutamyltransferase 1	Gamma-GT	5.6
8	(GDP-D-MANNOSE dehydratase	GMD	3.5
8	Glycolipid transfer protein	GLTP	2.6
8	Lipase		3.8
8	Lysophosphatidic acid acyltransferase-b	LPAAT- b	3.4
8	Malonyl-CoA decarboxylase	MLYCD	4.8
8	NTPDase 3; CD39L 3	ENTPD3	7.2
8	Pancreatic isozyme	Glucokinase	3.4
8	Phosphate regulating neutral endopeptidase	NEP	11
8	Putative NAD (P)-dependent cholesterol dehydrogenase	MDH	3
8	Red cell phosphatase1, isozyme F	ACP1	2.3
	Down-regulated genes1
1	Insulin precursor		2.8
1	IG GAMMA-3 chain C region	HDC	2
1	Interlaken 1 receptor 1 precursor	IL-1R, IL-1Rα	3.8
1	Growth regulated protein (neutrophil-activating protein 3)	NAP-3	2.3
1	Natural killer cells antigen CD94	KP43	4.2
1	Monocyte chemotactic protein 1		4.3
1	T-cell surface glycoprotein CD3 e chain precursor		5.2
1	Heparin-binding growth factor 1		7.3
2	EGF-containing fibulin-like extracellular matrix protein 1	FIBL-3	2.1
2	Homocontig12		2.3
2	MT4-MMP		2
3	Glocosyl structure		2
3	Microtubule-associated protein 2	MAP2C	2.1
3	Mucosal addressin cell adhesion molecule-1		2.1
3	Peripheral myelin protein 22	PMP-22	3.8
4	Growth factor regulated channel 5	GRC5	2.1
4	Growth hormone secretagogue receptor type 1		2
4	a platelet-derived growth factor receptor (CD104A antigen)	PDGFRa	2.1
4	Glucose transporter type 5, small intestine (fructose transporter)		2.2
4	Dopamine receptor		2.3
4	C-C chemokine receptor type 9	CCR-9	2.4
4	Monocarboxylate-transporter 3	MCT 3	2.9
4	Orphan receptor	GRF	3.6
4	G protein-coupled receptor	GRP1	3.6
4	Neuropeptide Y Receptor Type 4	NPY4-R	10.6
4	b 3 Adrenergic Receptor		2.5
5	G25K GTP-binding protein, placental isoform (GP)		2
5	MAD 2		2
5	Ribosomal S6 protein kinase		2
5	Metalloproteinase Inhibitor 3	TIMP-3	2.2
5	GTPase RhoD	RhoD	2.4
5	Ankyrins		2.7
5	Putative RHO/RAC effector protein		4.2
5	Protein farnesyltransferase β subunit (RAS Proteins prenyltransferase)	FTASE-β	2.4
6	GTP-binding protein	RHEB	2.2
6	GTP-binding protein 1	GP-1	2.3
6	hLHX 6 1α		2
6	AMP-binding protein		2.7
7	Thyroid peroxidase	TPO	2.1
8	Ubiquitin-conjugating enzyme E2 (ubiquitin-protein ligase)	UBE2	2
8	Steryl-sulfatase (steroid sulfatase) (steryl-sulfate sulfhydrolase)	ASC	2
8	Histone deacetylase 2	HD2	2.1
8	Corticosteroid 11-β-dehydrogenase, isozyme 2		4.3
8	Hydroxymethylglutaryl-CoA synthase		4.4
8	ATP synthase oligomycin sensitivity conferral protein	OSCP	4.9
8	Indoleamine 2,3-dioxygenase	IDO	2.1
8	Lysozyme		2.2
8	ubiquitin C-terminal hydrolase	UCH	2.3
8	Tyrosine aminotransferase,L-tyrosine: 2-oxoglutarate Aminotransferase	TAT	2.7
8	Corticosteroid 11-β-dehydrogenase, isozyme 2		4.3
8	Hydroxymethylglutaryl-CoA synthase		4.4
8	ATP synthase oligomycin sensitivity conferral protein	OSCP	4.9
8	Glycogen phosphorylase		8.5

¹52 selected genes (n = 3/group, study repeated × 2). Dibutyltin dichloride induced pancreatitis regulated genes (up or down-regulated) expression normalized with sham controls. Group 1: Circulatory/acute phase/immunomodulatory; Group 2: Extracellular Matrix; Group 3: Structural; Group 4: Channel/receptor/transporter; Group 5: Signaling transduction; Group 6: Transcription/translation-related; Group 7: Antioxidants/chaperones/heat shock; Group 8: Pancreatic and other enzymes; Group 9: Unidentified genes. Total genes = 321; up-regulated = 261, down-regulated = 60.