Uncoupling protein 2 regulates glucagon-like peptide-1 secretion in L-cells

Figure 3 Expression of uncoupling protein-2 in NCI-H716 cells after treatment with oleic acid. A: Total RNA was extracted, and relative mRNA levels of uncoupling protein-2 (UCP2) were determined by quantitative reverse transcriptase polymerase chain reaction with normalization to β-actin (P < 0.05); B: UCP2 protein was detected by western blotting. Cytochrome C was used as the loading control (P < 0.01); Individual data points in this figure represent the mean ± SE of 12 determinants from four independently prepared samples each with three measurements. NCI-H716 cells were incubated with medium alone (blank control), medium containing 1% dimethyl sulfoxide (negative control), medium with 30 mmol glucose (glucose), and medium with 8 mmol oleic acid (oleic acid).