Function of chloride intracellular channel 1 in gastric cancer cells

Figure 2 Effect of chloride intracellular channel 1 knockdown in SGC-7901 and MGC-803 cells. Chloride intracellular channel 1 (CLIC1) protein expression was detected by Western blotting using a rat polyclonal CLIC1 antibody (1:500). β-actin was used as loading control. CLIC1 mRNA expression was detected by quantitative real-time polymerase chain reaction. β-actin was used as loading control. The figure represents one of the three experiments performed independently with similar results. A: CLIC1 protein expression in SGC-7901 by Western blotting; B: CLIC1 protein expression in MGC-803 by Western blotting; C: Relative CLIC1 protein expression level in SGC-7901; D: Relative CLIC1 protein expression level in MGC-803; E: Relative CLIC1 mRNA expression level in SGC-7901; F: Relative CLIC1 mRNA expression level in MGC-803. The experiment was done in triplicate and the value obtained from the control (CTRL) group set as 100%. ^aP < 0.05, ^bP < 0.01 vs CTRL and mock.