Knockdown of liver-intestine cadherin decreases BGC823 cell invasiveness and metastasis in vivo

doi:10.3748/wjg.v18.i24.3129

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Jun 28, 2012 (publication date) through Apr 25, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 28, 2012; 18(24): 3129-3137
Published online Jun 28, 2012. doi: 10.3748/wjg.v18.i24.3129

Table 1 Altered proteins in lenti-liver-intestine cadherin-miR-B compared with BGC823 cells

Protein description	Gene name	Accessionno.¹	MW/pI(theo)¹	Sequencecoverage²(%)	Mascotscore³	MS/MS peptide sequence⁴	Cellular location
Clathrin light chain A	CLCA	P09496	27 174/4.43	15	59	K.AIKELEEWYAR.Q	Cytoplasmic vesicle membrane; peripheral membrane; cytoplasm
Nucleophosmin	NPM	Q96AT6	32 726/4.64	23	56	K.MSVQPTVSLGGFEITPPVVLR.L	Nucleus; nucleoplasm
Tropomyosin α-3 chain	TPM3	P06753	32 856/4.68	7	56	R.KLVIIEGDLER.T	Cytoplasm; cytoskeleton
Tubulin beta-2C chain	TBB2C	P68371	50 255/4.79	19	61	R.AVLVDLEPGTMDSVR.S	Cytoplasm
Glutathione S-transferase P	GSTP1	P09211	23 569/5.43	43	298	M.PPYTVVYFPVR.G	Nucleus; cytoplasm
Activator of 90 kDa heat shock protein ATPase homolog 1	AHSA1	O95433	38 421/5.41	30	143	K.ETFLTSPEELYR.V	Cytoplasm; endoplasmic reticulum
Interleukin-1 α	IL1A	P01583	30 758/5.04	23	64	K.LTFKESMVVVATNGK.V	Secreted
Heat shock 70 kDa protein 1A/1B	HSP71	P08107	70 294/5.48	27	239	K.DAGVIAGLNVLR.I	Cytoplasm
78 kDa glucose-regulated protein	GRP78	P11021	72 402/5.07	18	100	R.ITPSYVAFTPEGER.L	Endoplasmic reticulum lumen; melanosome
Actin, cytoplasmic 1	ACTB	Q96HG5	42 052/5.29	36	210	R.AVFPSIVGRPR.H	Cytoplasm; cytoskeleton
Pyruvate kinase isozymes M1/M2	KPYM	Q9BWB5	58 470/7.96	30	161	R.LDIDSPPITAR.N	Cytoplasm; nucleus
Mitochondrial 28S ribosomal protein S22	RT22	P82650	41 425/7.70	21	131	K.YVFTDISYSIPHR.E	Mitochondria
NADH dehydrogenase (ubiquinone) iron-sulfur protein 2, mitochondrial	NDUS2	O75306	52 911/7.21	25	86	K.TYLQALPYFDR.L	Mitochondrial inner membrane; peripheral membrane; matrix side
Vinculin	VINC	P18206	124 292/5.50	26	183	R.EAFQPQEPDFPPPPPDLEQLR.L	Cytoplasm; cytoskeleton; cell membrane; peripheral membrane
α-enolase	ENOA	Q6GMP2	47 481/7.01	29	134	R.AAVPSGASTGIYEALELR.D	Cytoplasm; cell membrane; myofibril; sarcomere; m-band

¹Accession Number. Theoretical molecular weight (MW, kDa) and pI were derived from the SWISS-PROT protein database;

²Sequence coverage of the matched peptides in the protein;

³Protein score (based on combined mass and mass/mass spectra) were from MALDI-TOF/TOF identification;

⁴Each spot corresponding to one protein had at least one of the shown peptides identified.

Citation: Xu Y, Zhang J, Liu QS, Dong WG. Knockdown of liver-intestine cadherin decreases BGC823 cell invasiveness and metastasis in vivo. World J Gastroenterol 2012; 18(24): 3129-3137
URL: https://www.wjgnet.com/1007-9327/full/v18/i24/3129.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i24.3129