Proteome profiling of spinal cord and dorsal root ganglia in rats with trinitrobenzene sulfonic acid-induced colitis

Table 1 Significantly regulated proteins after trinitrobenzene sulfonic acid-induced colitis in dorsal root ganglia

Function	Cell component	Protein name	Abbreviation	AccessionNo.	TheoreticalPI/Mr (kDa)	SequenceCoverage (%)	MASSOT score	Change(TNBS)
Proteins involved in inflammatory/immune response
Autoantigen in many autoimmune diseases	Cytoplasm, nucleus spliceosome	Isoform B0a of heterogeneous nuclear ribonucleoproteins A2/B1	Hnrnpa2b1	IPI00923129	8.74/32 572	16	220	↑
Hemopexin	Secreted	Hemopexin	Hpx	IPI00195516	7.58/52 060	22	597	↑
1. Accelerate the folding of protein 2. Immunosuppression	Cytosol, nucleus	Peptidyl-prolyl cis-trans isomerase A	Ppia	IPI00387771	8.34/18 091	32	363	↓
Proteins involved in cell signaling
Growth protein	Membrane	Adenylyl cyclase-associated protein 1	Cap1	IPI00555187	7.16/51 899	5	145	↑
Cytoplasmic tetramerisation domain of voltage-gated K+ channel		Similar to potassium channel tetramerisation domain containing protein 12	Kctd12	IPI00359734	8.95/47 077	16	192	↑
1. Ion channel 2. Mitochondrial apoptosis	Mitochondrial outer membrane	Voltage-dependent anion-selective channel protein 2	Vdac2	IPI00198327	7.44/32 353	9	76	↓
Proteins involved in redox regulation
Cell redox homeostasis	Mitochondria	Dihydrolipoyl dehydrogenase, mitochondrial	Dld	IPI00365545	7.96/54 574	5	141	↑
Antioxidant	Mitochondria	Superoxide dismutase [Mn], mitochondrial	Sod2	IPI00211593	8.96/24 887	22	130	↓
Xenobiotic metabolism and cellular defense	Nucleus	Glutathione S-transferase P	Gstp1	IPI00231229	6.89/23 652	12	255	↓
Eliminating peroxides	Mitochondria, cytosol	Peroxiredoxin-1	Prdx1	IPI00211779	8.27/22 323	9	108	↓
Proteins involved in chaperone function
Chaperonins/heat shock proteins	Mitochondria	Heat shock cognate 71 kDa protein	Hspa8	IPI00208205	5.37/71 055	18	304	↓
Chaperonins/heat shock proteins	Nucleus, cytoplasm	Stress-induced-phosphoprotein 1	Stip1	IPI00213013	6.40/63 158	9	156	↑
Proteins involved in cellular structure
Component of the nuclear lamina	Insoluble fraction, lamin filament, nuclear matrix	Lamin C2	Lmna	IPI00188879	6.22/52 661	16	136	↑
Proteins involved in cellular metabolism
Oxidoreductase in valine and pyrimidine catabolic pathways	Mitochondria	Methylmalonate-semialdehyde dehydrogenase [acylating],mitochondrial	Aldh6a1	IPI00205018	8.44/58 396	4	68	↓
Glycolytic enzyme	Mitochondria	Fructose-bisphosphate aldolase A	Aldoc	IPI00231734	8.31/39 783	11	148	↓
Glycolytic enzyme	Axon, mitochondria	Aldoc 17 kDa protein	Aldoc	IPI00561972	6.84/16 666	14	88	↓
Glycolytic enzyme	Mitochondria	Dihydrolipoyllysine-residueacetyltransferase component of pyruvate dehydrogenase complex	Dlat	IPI00231714	8.76/67 637	7	79	↑
Glycolytic enzyme	Cytoplasm	Phosphoglycerate kinase 1	Pgk1	IPI00231426	8.02/44 909	16	184	↓
Glycolytic enzyme		Similar to pyruvate kinase 3		IPI00561880	7.15/58 264	17	231	↓
Glycolytic enzyme	Nucleus cytoplasm	Isoform M1 of pyruvate kinase isozymes M1/M2	Pkm2	IPI00231929	6.63/58 452	22	491	↑
1. Glycolytic enzyme 2. Transcription activation 3. Initiation of apoptosis	Cytoplasm	Similar to glyceraldehyde-3-phosphate dehydrogenase	RGD1565368	IPI00554039	8.44/36 045	14	347	↑
ATP energy transduction	Cytoplasm	Creatine kinase M-type	Ckm	IPI00211053	6.58/43 246	19	361	↑
ATP transducing	Mitochondria	Creatine kinase, mitochondrial 1, ubiquitous	Ckmt1	IPI00555166	8.58/47 331	21	231	↓
TCA cycle	Mitochondria	Isoform mitochondrial of fumarate hydratase, mitochondrial	Fh1	IPI00231611	9.06/54 714	5	115	↓
1. TCA cycle 2. Gluconeogenesis 3. Antioxidant	Mitochondria	Malate dehydrogenase, mitochondrial	Mdh2	IPI00197696	8.93/36 117	11	90	↓
Extrahepatic ketone body catabolism	Mitochondria	3-ketoacid-coenzyme A transferase 1, mitochondrial	Oxct1 Succinyl-CoA	IPI00766788	8.7/56 624	6	116	↑

↑: Elevated protein expression in TNBS group compared with saline group; ↓: Decreased protein expression in TNBS group in comparison with saline group. DRG: Dorsal root ganglia; TNBS: Trinitrobenzene sulfonic acid; Hnrnpa2b1: Heterogeneous nuclear ribonucleoproteins A2/B1; Cap1: Cyclase-associated protein 1; Kctd12: Potassium channel tetramerisation domain containing protein 12; Vdac2: Voltage-dependent anion-selective channel protein 2; Dld: Dihydrolipoyl dehydrogenase; Sod2: Superoxide dismutase; Gstp1: Glutathione S-transferase P; Prdx1: Peroxiredoxin-1; Stip1: Stress-induced phosphoprotein 1; Lmna: Lamin C2; Ckm: Creatine kinase M-type; Ckmt1: Creatine kinase, mitochondrial 1, ubiquitous; Fh1: Fumarate hydratase; Mdh2: Malate dehydrogenase; Oxct1 Succinyl-CoA: 3-ketoacid-coenzyme A transferase 1.