Anti-tumor effect of 5-aza-2'-deoxycytidine by inhibiting telomerase activity in hepatocellular carcinoma cells

Table 1 Primer sequences for reverse transcription-polymerase chain reaction and methylation-specific polymerase chain reaction analysis

Gene	Primer sequence (5’-3’)	Annealing(°C)	Product size (bp)
hTERT-F	CGGAAGAGTGTCTGGAGCAA	58	422
hTERT-R	GGATGAAGCGGAGTCTGGA
c-myc-F	CTTCTCTCCGTCCTCGGATTCT	65	132
c-myc-R	GAAGGTGATCCAGACTCTGACCTT
p15-F	GAATGCGCGAGGAGAACAAG	65	204
p15-R	CCATCATCATGACCTGGATCG
p16-F	GCTGCCCACGCACCGAATA	57	179
p16-R	ACCACCAGCGTGTCCAGGAA
p21-F	GCAGACCAGCATGACAGATTT	60	70
p21-R	GGATTAGGGCTTCCTCTTGGA
WT1-F	GGCATCTGAGACCAGTGAGAA	62	120
WT1-R	GAGAGTCAGACTTGAAAGCAGT
E2F1-F	AGCTGGACCACCTGATGAAT	60	95
E2F1-R	GTCCTGACACGTCACGTAGG
β-actin-F	CCTGTACGCCAACACAGTGC	60	275
β-actin-R	ATACTCCTGCTTGCTGATCC
hTERT-MF	AGTTTTGGTTTCGGTTATTTTCGC	58	122
hTERT-MR	AACGTAACCAACGACAACACC
hTERT-UF	AGTTTTGGTTTTGGTTATTTTTGT	58	132
hTERT-UR	AACGTAACCAACGACAACACCT
p16-MF	TTATTAGAGGGTGGGGCGGATCGC	56	150
p16-MR	GACCCCGAACCGCGACCGTAA
p16-UF	TTATTAGAGGGTGGGGTGGATTGT	56	151
p16-UR	CAACCCCAAACCACAACCATAA