CD74 and macrophage migration inhibitory factor as therapeutic targets in gastric cancer

Figure 3 Lipopolysaccharide stimulation induced migration inhibitory factor and surface CD74 expression in gastric cancer cell line MKN45. A: MKN-45 cells were transduced with control or CD74-specific shRNA, and the percentage of GFP⁺ cells is shown before or after flow cytometry sorting; B: CD74 expression was measured by western blotting when the MKN-45 cells were infected by control or CD74-specific shRNA; C and D: MKN-45 cells were knocked down for CD74 and stimulated with lipopolysaccharide (LPS) (C) or migration inhibitory factor (MIF) (D) for 24 h; cell proliferation was measured by CCK8; E and F: MKN-45 cells were stimulated with LPS at 1 μg/mL, and blocked with MIF antagonist ISO-1 (E) or CD74 antibody (F) for 24 h, and cell proliferation was measured by CCK8. GFP: Green fluorescent protein.^aP < 0.05, ^bP < 0.01.