Pathogenesis of NSAID-induced gastric damage: Importance of cyclooxygenase inhibition and gastric hypermotility

Figure 13 Gastric ulcerogenic effect of indomethacin, rofecoxib and SC-560 and the expression of cyclooxygenase-1 and cyclooxygenase-2 mRNA in the gastric mucosa of normal and arthritic rats. A: Arthritis was induced by injecting Freund’s complete adjuvant (FCA) into the plantar region of the right hindfoot, and the experiments were performed 14 d after the injection. Indomethacin (Indo) (3 mg/kg), rofecoxib (Rof) (30 mg/kg), or SC-560 (SC) (30 mg/kg) were administered p.o., and the animals were killed 4 h later. Data are presented as the mean ± SE in 4-8 animals, ^aP < 0.05 vs the corresponding group in normal rats; B: COX-2 mRNA was not detected in the normal rats, but clearly observed in the arthritic rats on day 14 after the FCA injection, whereas COX-1 mRNA was observed in the stomach of both normal and arthritic rats. Lane 1, marker; lanes 2-4, normal rats; lanes 5-7, arthritic rats (data from ref. 23 after modification). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.