Affinity peptide developed by phage display selection for targeting gastric cancer

Figure 5 Binding analysis of biotin-AADNAKTKSFPV by immunohistochemistry. The results demonstrate that biotin showed a specific binding affinity to gastric cancer (GC) (A: Intestinal; B: Diffuse). In contrast, no positive staining was observed in gastric mucosae (C). In addition, peptide AADNAKTKSFPV (AAD) did not bind to breast cancer (D) or colon cancer (E), suggesting that the AAD peptide is specific to GC. A small amount of binding of the peptide AAD to the gastric mucosa dysplasia (F) and intestinal metaplasia (G) was also observed. The negative results were also obtained when GC tissues were stained with the biotin-conjugated scramble peptide (H) or phosphate-buffered saline (I).