Transactivation of the TIEG1 confers growth inhibition of transforming growth factor-β-susceptible hepatocellular carcinoma cells

Figure 3 Downregulation of transforming growth factor-β-inducible early gene 1 by RNA interference (siRNA) decreased the transforming growth factor-β1 susceptibility of Hep3B cells. A: Decreases in the transforming growth factor (TGF)-β-inducible early gene 1 (TIEG1) mRNA level in siRNAs targeting TIEG1 (siTIEG1)-transfected Hep3B cells with or without TGF-β1 treatment. Cells were transfected with siTIEG1 prior to the TGF-β1 treatments described above. After 4 h of treatment, mRNA levels of TIEG1 were determined by quantitative reverse transcriptase-polymerase chain reaction; B: Increases in the survivability of the siTIEG1-transfected and the TGF-β1-treated Hep3B cells. The decreased growth inhibitory response of Hep3B cells to TGF-β1 treatment was determined by methylthiazoletetrazolium assays after 72 h of TGF-β1 treatment. ^aP < 0.05.