Overexpression of Dickkopf-3 induces apoptosis through mitochondrial pathway in human colon cancer

Figure 4 Overexpression of Dickkopf-3 inhibits proliferation and induces G0/G1 arrest in human colon cancer LoVo cells. A: Dickkopf-3 (Dkk-3) inhibits proliferation in human colon cancer LoVo cells; B: Forty-eight hours after transfection, LoVo was used for cell cycle analysis using a FACSCalibur flow cytometer; C: The dickkopf homolog 3 gene Dkk-3 inhibited tumor cell colony formation. LoVo cells were transfected with pEGFP-N1-Dkk-3-GFP plasmid or with pEGFP-N1 and were maintained in the presence of G418 sulfate for 2 wk. Quantitative analysis of colony numbers are shown as the mean ± SD. ^aP < 0.05 vs pEGFP-N1 using Student’s t test.