Increased presence of effector lymphocytes during Helicobacter hepaticus-induced colitis

Figure 3 Changes in T lymphocyte populations and natural killer cells in the mesenteric lymph nodes of drosophila mothers against decapentaplegic 3^+/- and drosophila mothers against decapentaplegic^-/- mice following infection with Helicobacter hepaticus. Flow cytometric analysis of lymphocyte populations at days 0, 3, 7 and 28 post-infection. Gates were drawn on viable cells using forward scatter vs side scatter parameters. A: Total CD3⁺ lymphocytes gated on forward scatter vs CD3; B: Total CD8⁺ lymphocytes gated on CD3⁺ lymphocytes; C: Total CD4⁺ lymphocytes gated on CD3⁺ lymphocytes; D: Total CD25⁺/FOXP3⁺ Treg cells gated on CD3⁺/CD4⁺ lymphocytes; E: Total natural killer (NK)p46⁺/DX5⁺ NK cells in mesenteric lymph nodes (n = 4-6 animals per time point). ^aP < 0.05 vs baseline values; ^cP < 0.05 denotes significant interaction between genotypes [drosophila mothers against decapentaplegic (SMAD)3^-/-vs SMAD3^+/-].