Strong expression of CD133 is associated with increased cholangiocarcinoma progression

Figure 4 Cell proliferation assays of CD133+ and CD133- RMCCA1 cells. A: The proliferation rates of CD133⁺ and CD133^- cholangiocarcinoma cells. CD133⁺ and CD133^- RMCCA1 cells were cultured for 24-72 h. A cell proliferation assay was performed using WST-1. Results are reported as a percentage of cell proliferation, where the optical density values from CD133⁺ cells cultured for 24 h are set as 100% of proliferation. Data is represented as the mean ± SD of 3 independent experiments. (^aP = 0.095, ^bP = 0.578, ^cP = 0.544 vs the same time of culturing); B: Effect of cisplatin on cholangiocarcinoma cells. CD133⁺ and CD133^- RMCCA1 cells were treated with cisplatin at various concentrations (0, 5, 10 and 50 μmol/L) for 2 d. Effects on cell proliferation were measured by WST-1. Results are reported as percentage of cell proliferation, where the optical density values from vehicle-treated cells (0 μmol/L cisplatin) are set as 100% proliferation. Data is presented as the mean ± SD of 3 independent experiments. (^aP = 0.060, ^bP = 0.056, ^cP = 0.053 vs the same concentration of cisplatin). WST: Water soluble tetrazolium salts.