Strong expression of CD133 is associated with increased cholangiocarcinoma progression

Figure 3 Isolation of CD133⁺ and CD133^- cholangiocarcinoma cells. A: The percentage of CD133^-expressing cells in the original RMCCA1 cell populations, the flushed-out fractions (CD133⁺ RMCCA1 cells) and the flow-through (CD133^- RMCCA1 cells) were analyzed by fluorescence-activated cell sorting; B: The morphology of original, CD133⁺ and CD133^- RMCCA1 cells is demonstrated under a phase contrast microscope at 200 × magnification. FITC: Fluorescein isothiocyanate.