Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells

Figure 3 Verification of mast-cell microarray results by quantitative reverse transcriptase-polymerase chain reaction with seven selected genes that are involved in mast-cell immune system regulation (A) and mast-cell activation (B). Gene expression was quantified after 24 h stimulation with four probiotic bacteria; Lactobacillus rhamnosus GG (LGG), Lactobacillus rhamnosus Lc705 (Lc705), Propionibacterium freudenreichii ssp. shermanii JS (PJS), Bifidobacterium animalis ssp. lactis Bb12 (Bb12) and their combination or with Chlamydia pneumoniae isolate Kajaani 6 (Cpn). Data are mean values ± SE of three independent experiments. IL: Interleukin; TNF-α: Tumor necrosis factor-α; CCL2: Chemokine (C-C motif) 2; FCER1A: Fc fragment of IgE high affinity I receptor for α polypeptide; FCER1G: Fc fragment of IgE high affinity I receptor for γ polypeptide; HRH4: Histamine H4 receptor.