Clinical significance of K-ras and BRAF mutations in Chinese colorectal cancer patients

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Feb 14, 2011 (publication date) through Apr 24, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 14, 2011; 17(6): 809-816
Published online Feb 14, 2011. doi: 10.3748/wjg.v17.i6.809

Table 2 Primers designed for amplification and pyrosequencing assay of K-ras and BRAF genes

	Primer sequence	Product (bp)
K-ras gene codon 12 & 13 (exon 1)	Forward: 5'-GCAGTCAACTGGAATTTTCATG-3'	431
	Reverse: 5'-biotin-GAAACCCAAGGTACATTTCAGA-3'
	Pyrosequencing assay: 5'-TGTGGTAGTTGGAGCT- 3'
K-ras gene codon 61 (exon 2)	Forward:5'-ATCCAGACTGTGTTTCTCCCTTC-3'	378
	Reverse: 5'-biotin-ACTGCTCTAATCCCCCAAGAACT-3'
	Pyrosequencing assay: 5'-TATTCACGACACAGCAGGT-3'
BRAF gene codon 600 (exon 15)	Forward: 5'-ACAAGCCTTCAAAAATGAAGTAG-3'	362
	Reverse: 5'-biotin-ATCCAGACAACTGTTCAAACTGA-3'
	Pyrosequencing assay: 5'-GGTGATTTTGGTCTAACTACA-3'

Citation: Shen H, Yuan Y, Hu HG, Zhong X, Ye XX, Li MD, Fang WJ, Zheng S. Clinical significance of K-ras and BRAF mutations in Chinese colorectal cancer patients. World J Gastroenterol 2011; 17(6): 809-816
URL: https://www.wjgnet.com/1007-9327/full/v17/i6/809.htm
DOI: https://dx.doi.org/10.3748/wjg.v17.i6.809