Erlotinib inhibits progression to dysplasia in a colitis-associated colon cancer model

Figure 7 Effects of erlotinib on RIE-1 cells. A: Cells were incubated in RPMI 1640-5% FBS with or without EGF (10 ng/mL) and the indicated concentrations of erlotinib for 24 h. Cells were also pretreated with indicated concentration of erlotinib for 24 h and then stimulated with EGF (10 ng/mL) for 5 min. Cell lysates were analyzed by immunoblotting with indicated antibodies; B and C: Cell proliferation was assayed in the presence or absence of EGF (10 ng/mL) or erlotinib (10 μmol/L) as described in the Materials and Methods. EGF: Epidermal growth factor.