Erlotinib inhibits progression to dysplasia in a colitis-associated colon cancer model

Figure 5 Effect of erlotinib in an animal model of colitis-associated dysplasia on mitogen-activated protein kinase components. Samples from (A) proximal, (B) mid, and (C) distal regions of normal rats and trinitrobenzene sulfonic acid (TNBS) + vehicle and TNBS + erlotinib treated animals were ground, and equal amounts of protein (30 μg) were separated by sodium dodecyl sulfate 12%-polyacrylamide gel electrophoresis before analysis by Western blotting. Antibodies against pErk1/2 and Erk1/2 were used. Lines are representative samples of 3 independent rats per group. Densitometry was performed by ImageQuant 5.2 Software (Typhoon 9410) (n = 10-20 ± SEM).