Plasma DNA methylation of Wnt antagonists predicts recurrence of esophageal squamous cell carcinoma

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Nov 28, 2011; 17(44): 4917-4921
Published online Nov 28, 2011. doi: 10.3748/wjg.v17.i44.4917

Table 1 Primers for methylation-specific polymerase chain reaction

Gene		Primer sequence (5'–3')
WIF-1
U	F	GGGTGTTTTATTGGGTGTATTGT
	R	AAAAAAACTAACACAAACAAAATACAAAC
M	F	CGTTTTATTGGGCGTATCGT
	R	ACTAACGCGAACGAAATACGA
RUNX-3
U	F	TTATGAGGGGTGGTTGTATGTGGG
	R	AAAACAACCAACACAAACACCTCC
M	F	TTACGAGGGGCGGTCGTACGCGGG
	R	AAAACGACCGACGCGAACGCCTCC
SFRP-1
U	F	GAGTTAGTGTTGTGTGTTTGTTGTTTTGT
	R	CCCAACATTACCCAACTCCACAACCA
M	F	GTGTCGCGCGTTCGTCGTTTCGC
	R	AACGTTACCCGACTCCGCGACCG
DKK-3
U	F	TTAGGGGTGGGTGGTGGGGT
	R	CTACATCTCCACTCTACACCCA
M	F	GGGGCGGGCGGCGGGGC
	R	ACATCTCCGCTCTACGCCCG

M: Methylated sequence; U: Unmethylated sequence; F: Forward primer; R: Reverse primer.

Citation: Liu JB, Qiang FL, Dong J, Cai J, Zhou SH, Shi MX, Chen KP, Hu ZB. Plasma DNA methylation of Wnt antagonists predicts recurrence of esophageal squamous cell carcinoma. World J Gastroenterol 2011; 17(44): 4917-4921
URL: https://www.wjgnet.com/1007-9327/full/v17/i44/4917.htm
DOI: https://dx.doi.org/10.3748/wjg.v17.i44.4917

Liu JB, Qiang FL, Dong J, Cai J, Zhou SH, Shi MX, Chen KP, Hu ZB. Plasma DNA methylation of Wnt antagonists predicts recurrence of esophageal squamous cell carcinoma. World J Gastroenterol 2011; 17(44): 4917-4921 [PMID: 22171134 DOI: 10.3748/wjg.v17.i44.4917]

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