Nitric oxide-releasing aspirin but not conventional aspirin improves healing of experimental colitis

Table 1 Primer sequence used in reverse transcription polymerase chain reaction determination of gene expression of proinflammatory factors

Gene		Primer
β-actin	Upstream	5’-TTG TAA CCA ACT GGG ACG ATA TGG-3’
	Downstream	5’-GAT CTT GAT CTT CAT GGT GCT AGG-3’
IL-1β	Upstream	5’-GCT ACC TAT GTC TTG CCC GT-3’
	Downstream	5’–GAC CAT TGC TGT TTC CTA GG-3’
TNF-α	Upstream	5’-TAC TGA ACT TCG GGG TGA TTG GTC C-3’
	Downstream	5’–CAG CCT TGT CCC TTG AAG AGA ACC-3’
COX-2	Upstream	5’–ACA ACA TTC CCT TCC TTC-3’
	Downstream	5’–CCT TAT TTC CTT TCA CAC C-3’
iNOS	Upstream	5’–CCA CAA TAG TAC AAT ACT ACT TGG–3’
	Downstream	5’–ACG AGG TGT TCA GCG TGC TCC ACG–3’

TNF: Tumor necrosis factor; COX: Cyclooxygenase; iNOS: Inducible form of nitric oxide synthase.