Late SV40 factor: A key mediator of Notch signaling in human hepatocarcinogenesis

Figure 2 Late SV40 factor expression is increased in human normal cells showing forced overexpression of exogenous Notch1-ICD. A: Transient transfection of H-Ras and/or NICD1 and stable transfection of H-Ras and/or NICD1 into hepatic stellate cells (HSC); B: Western-blotting analysis of H-Ras and NICD1 from human embryonic kidney (HEK) cells and human HSC; C: Late SV40 factor (LSF)-dependent expression correlates with Notch1-ICD protein levels in human normal cells after forced overexpression of exogenous Notch1-ICD. Co-expression of H-Ras and Notch1-ICD significantly increases LSF levels.